





In The IMP lab, we employ a broad range of immunological, molecular and biochemical techniques to execute our experiments
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Animal
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Modeling Human Cancer in Mice , intraperitoneal and S.C.injections, animal surgery
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Cell Culture
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Primary cell Isolation, Stem cells and immortalized cell line maintenance and manipulation, In vitro cytotoxicity studies, migration and invasion assay.
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Protein
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E. coli / mammalian cell system for protein expression and structure characterization/determination “NMR and Circular Dichroism”, membrane protein stability using micelles and bicelles, in Silica molecule/protein-Receptor interaction by High Ambiguity Driven Biomolecular DOCKing (HADDOCK), SDS-PAGE, Western blot analysis, Isoelectric focusing strips, and 2D protein gels.
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Nucleic Acid
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Aptamer developments, DNA / RNA isolation and purification, agarose gel electrophoresis, PCR, qPCR, Next Gen Sequencing,siRNA/shRNA knockdowns, microRNA target gene analysis and pathway identification.
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Engineering cells with CRISPR, Design Donor DNA and gRNA Nuclease, Nucleofection, Gene Knock Out Using Targeted Nucleases.
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Flow Cytometry
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Sample preparation for flow cytometry, multicolor cell staining, compensation and analysis.
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Fluorescence microscope